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xfecttm polymer  (TaKaRa)


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    Structured Review

    TaKaRa xfecttm polymer
    Xfecttm Polymer, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xfect+polymer/Xfect+Transfection+Reagent/us12612448-180-34-36
    Average 96 stars, based on 1309 article reviews
    xfecttm polymer - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymer:

    Article Title: U1 RNA Detected by Toll-Like Receptor 3 Plays a Role in the Pathogenesis of Pterygium
    Article Snippet: .. Cell lysates, except for one group (UVB-irradiated cell lysate without Xfect polymer), were cotreated with Xfect polymer (631318; Clontech Laboratories) to enhance dsRNA entry. .. After treatment, cells were lysed with a protein extraction solution containing protease inhibitors, and protein concentrations were measured using the bicinchoninic acid assay (Thermo Fisher Scientific Waltham, MA, USA), with bovine serum albumin as the standard.

    Article Title: Ubiquitin regulatory X (UBX) domain-containing protein 6 is essential for autophagy induction and inflammation control in macrophages.
    Article Snippet: .. BMDMs were primed with LPS (100 ng/mL) in Opti-MEM (Gibco, 31985- 070) for 4 h and then stimulated with 5mM ATP (Sigma‒Aldrich, A5394) or 10 μM nigericin sodium salt (Sigma‒Aldrich, SML1779) for 45min to activate the canonical inflammasome or transfected with 2 μg/mL LPS using Xfect polymer (Clontech Laboratories, Mountain View, CA, USA, 631318) according to the manufacturer’s instructions for the indicated times to activate the noncanonical inflammasome. ..

    Article Title: MRPL47 Deficiency Drives Mitochondrial Dysfunction via ROS/p38-MAPK/CDKN1A Signaling in Non-Small Cell Lung Cancer
    Article Snippet: .. Transfection was performed using Xfect polymer (TaKaRa Bio, cat.#631317) in accordance with the manufacturer’s guidelines. .. The transfected cells were selected with 2.5 μg/mL puromycin (Sigma, cat.#P8833) for at least 48 hours.

    Article Title: Ubiquitin regulatory X (UBX) domain-containing protein 6 is essential for autophagy induction and inflammation control in macrophages
    Article Snippet: .. BMDMs were primed with LPS (100 ng/mL) in Opti-MEM (Gibco, 31985-070) for 4 h and then stimulated with 5 mM ATP (Sigma‒Aldrich, A5394) or 10 μM nigericin sodium salt (Sigma‒Aldrich, SML1779) for 45 min to activate the canonical inflammasome or transfected with 2 μg/mL LPS using Xfect polymer (Clontech Laboratories, Mountain View, CA, USA, 631318) according to the manufacturer’s instructions for the indicated times to activate the noncanonical inflammasome. .. BMDMs were primed with LPS (100 ng/mL) in Opti-MEM (Gibco, 31985-070) for 4 h and then stimulated with 5 mM ATP (Sigma‒Aldrich, A5394) or 10 μM nigericin sodium salt (Sigma‒Aldrich, SML1779) for 45 min to activate the canonical inflammasome or transfected with 2 μg/mL LPS using Xfect polymer (Clontech Laboratories, Mountain View, CA, USA, 631318) according to the manufacturer’s instructions for the indicated times to activate the noncanonical inflammasome.

    Article Title: MRPL47 deficiency drives mitochondrial dysfunction via ROS-p38-p21 signaling in non-small cell lung cancer
    Article Snippet: .. Transfection was performed using Xfect polymer (TaKaRa Bio, cat.#631317) in accordance with the manufacturer's guidelines. .. The transfected cells were selected with 2.5 μg/ml puromycin (Sigma, cat.#P8833) for at least 48 h. For siRNA mediated RNA interference, cells were transfected with 5 nM Ambion Silencer Negative Control #1 siRNA (Thermo Fisher Scientific, cat #AM4611) and MRPL47 siRNA (siMRPL47) utilizing Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, cat #13778100), in accordance with the manufacturer's guidelines.

    Transfection:

    Article Title: Ubiquitin regulatory X (UBX) domain-containing protein 6 is essential for autophagy induction and inflammation control in macrophages.
    Article Snippet: .. BMDMs were primed with LPS (100 ng/mL) in Opti-MEM (Gibco, 31985- 070) for 4 h and then stimulated with 5mM ATP (Sigma‒Aldrich, A5394) or 10 μM nigericin sodium salt (Sigma‒Aldrich, SML1779) for 45min to activate the canonical inflammasome or transfected with 2 μg/mL LPS using Xfect polymer (Clontech Laboratories, Mountain View, CA, USA, 631318) according to the manufacturer’s instructions for the indicated times to activate the noncanonical inflammasome. ..

    Article Title: MRPL47 Deficiency Drives Mitochondrial Dysfunction via ROS/p38-MAPK/CDKN1A Signaling in Non-Small Cell Lung Cancer
    Article Snippet: .. Transfection was performed using Xfect polymer (TaKaRa Bio, cat.#631317) in accordance with the manufacturer’s guidelines. .. The transfected cells were selected with 2.5 μg/mL puromycin (Sigma, cat.#P8833) for at least 48 hours.

    Article Title: Ubiquitin regulatory X (UBX) domain-containing protein 6 is essential for autophagy induction and inflammation control in macrophages
    Article Snippet: .. BMDMs were primed with LPS (100 ng/mL) in Opti-MEM (Gibco, 31985-070) for 4 h and then stimulated with 5 mM ATP (Sigma‒Aldrich, A5394) or 10 μM nigericin sodium salt (Sigma‒Aldrich, SML1779) for 45 min to activate the canonical inflammasome or transfected with 2 μg/mL LPS using Xfect polymer (Clontech Laboratories, Mountain View, CA, USA, 631318) according to the manufacturer’s instructions for the indicated times to activate the noncanonical inflammasome. .. BMDMs were primed with LPS (100 ng/mL) in Opti-MEM (Gibco, 31985-070) for 4 h and then stimulated with 5 mM ATP (Sigma‒Aldrich, A5394) or 10 μM nigericin sodium salt (Sigma‒Aldrich, SML1779) for 45 min to activate the canonical inflammasome or transfected with 2 μg/mL LPS using Xfect polymer (Clontech Laboratories, Mountain View, CA, USA, 631318) according to the manufacturer’s instructions for the indicated times to activate the noncanonical inflammasome.

    Article Title: MRPL47 deficiency drives mitochondrial dysfunction via ROS-p38-p21 signaling in non-small cell lung cancer
    Article Snippet: .. Transfection was performed using Xfect polymer (TaKaRa Bio, cat.#631317) in accordance with the manufacturer's guidelines. .. The transfected cells were selected with 2.5 μg/ml puromycin (Sigma, cat.#P8833) for at least 48 h. For siRNA mediated RNA interference, cells were transfected with 5 nM Ambion Silencer Negative Control #1 siRNA (Thermo Fisher Scientific, cat #AM4611) and MRPL47 siRNA (siMRPL47) utilizing Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific, cat #13778100), in accordance with the manufacturer's guidelines.

    other:

    Article Title: Oncolytic virus improved in safety and anticancer effect
    Article Snippet: After 2 hours, the medium was replaced with EMEM medium containing 2% fetal bovine serum, and then the cells were transfected with 4 μg of the linearized shuttle plasmid vector as constructed in Example 1.1 by employing XfectTM polymer (Clontech 631317, USA).

    Article Title: U1 RNA Detected by Toll-Like Receptor 3 Plays a Role in the Pathogenesis of Pterygium.
    Article Snippet: After treatment, cells were lysed with a protein extraction solution containing protease inhibitors, and protein concentrations were measured using the bicinchoninic acid assay (Thermo Fisher Scientific Waltham, MA, USA), with bovine serum albumin as the standard.

    Article Title: MRPL47 Deficiency Drives Mitochondrial Dysfunction via ROS-p38-p21 Signaling in Non-Small Cell Lung Cancer.
    Article Snippet: This was accomplished by designing a 21 bp target sequence for MRPL47 and subsequently cloning it into the Age1-EcoR1 sites of the pLKO vector.



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